JMSSJ On-line

JMSSJ On-line, Vol. 44 (1996) No. 2, pp. 261-267

ESI/MS Studies on Macrolide Antibiotics Phosphorylated by an Extract of an Erythromycin Resistant-Escherichia coli		>> Full Text PDF >> References
*Yasuo SHIDA,^a) Motoko FUKUZAWA,^a) Keiko YAMAMOTO,^b) and Koji O’HARA^b)* ^a) Department of Chemical Analysis and^b) Department of Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Science (1432-1, Horinouchi, Hachioji, Tokyo 192-03, Japan)

Two macrolide antibiotics, clarithromycin and roxithromycin, have recently entered the market and are now in frequent clinical use. These antibiotics were inactivated by an inactivating enzyme, macrolide 2′-phosphotransferase [MPH(2′)I], extracted from highly erythromycin-resistant Escherichia coli, and the reaction products were analyzed by ESI-MS. It was demonstrated that the 2′-phosphate derivatives of these macrolides could be detected by analysis of the mixture of MPH(2′)I and adenosine 5′-triphosphate after only a simple sample pretreatment with the addition of 3% acetic acid into water-methanol (1 : 1, v/v).

Key words: ESI, MS, Macrolide antibiotic, EM, OL, CAM, RXM, Macrolide 2′-phosphotransferase