| Determination of Organic Constituents in Human Serum by Isotope Dilution Method Using Discharge-assisted Thermospray Liquid Chromatography Mass Spectrometry |
>> Full Text PDF
>> References |
|
* National Institute of Materials and Chemical Research (Tsukuba, Ibaraki 305, Japan) |
||
| We review recently developed isotope dilution methods using discharge-assisted thermospray (plasmaspray) liquid chromatography/mass spectrometry (LC/MS) for determination of total cholesterol, glucose, creatinine, and urea in human serum. We have optimized the analytical conditions and discussed the analytical performances of the LC/MS methods. With an optimal conditions, the relative standard deviation of LC/MS methods was between 0.5 and 1.3%. We could conclude that the LC/MS method has the advantage in its simplicity of sample preparation, compared with the GC/MS, and could be a useful technique in IDMS analysis. | ||
| Key words: Isotope dilution mass spectrometry, Discharge-assisted thermospray liquid chromatography mass spectrometry, Cholesterol, Glucose, Creatinine, Urea, Human serum | ||
| [ Full Text PDF ] [ References ] |
| © COPYRIGHT by The Mass Spectrometry Society of Japan. All Rights Reserved. |