| Know-How of the High Sensitivity Quantitative Analysis of Drugs by LC/API/MS |
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* Pharmaceutical and Pharmacokinetic Research Laboratories, Fujisawa Pharmaceutical Co., Ltd. (1-6 Kashima 2-chome, Yodogawa-ku, Osaka 532, Japan) |
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| LC/API/MS enables quantitative analysis of drugs with high sensitivity. In order to obtain fine mass spectra, know-how for high sensitivity analysis is required. A stable spray can produce stable ionization for LC/MS analysis. It is also necessary to purify the samples for quantitation with high sensitivity. In addition to those, adequate analytical conditions of HPLC, LC/API/MS interface, and SIM (or SRM/MS) are important for analysis. Fragmentations observed in EI, CI, FAB, and LC/ESI mass spectrometry gave us hints for the selections of mass numbers for SRM analysis. LC/MS/MS analysis of enables quatitation of pg/ml level of drugs in plasma. | ||
| Key words: Electrospray mass spectrometry, Quantitative analysis, FK506, LC/API/MS, LC/APCI/MS, LC/ESI/MS, LC/ESI/SRM/MS, LC/ESI/SIM/MS | ||
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