JMSSJ On-line

JMSSJ On-line, Vol. 45 (1997) No. 3, pp. 401-415

Quantitative Method for Biological Active Substance in Biological Samples by Hyphenated MS Techniques -What is the key point?-		>> Full Text PDF >> References
Nariyasu MANO^a), Yoshiya ODA^, and Naoki ASAKAWA^* ^a) Analytical Chemistry Section, Eisai Tsukuba Research Laboratories (5-1-3 Tokodai, Tsukuba, Ibaraki 300-26, Japan)*

Mass spectrometry (MS) has been recognized as an integral method in biological research and our ability to analyze a wide variety of biologically relevant molecules has been greatly advanced by MS. Highly sensitive, highly selective, and reproducible hyphenated MS (GC/MS, LC/MS) or MS/MS method is necessary to get high quality information. We show that the optimization of MS interface is very important for high sensitivity. Chromatography techniques are also indispensable to distinguish identical mass substances. In addition to avoiding adsorption and contaminants problem, our optimized conditions have resulted in a suitable quantitative analysis method for endogenous biological active substances in biological materials.

Key words: LC/MS, GC/MS, MS/MS, Column-switching, Quantitation, Pretreatment