JMSSJ On-line

JMSSJ On-line, Vol. 47 (1999) No. 3, pp. 168-182

High Sensitive Analysis of Modified Nucleosides by LC/MS Using ESI/Iontrap Mass Spectrometry		>> Full Text PDF >> References
*Tsutomu SUZUKI^a), Takeo SUZUKI^a), Takuya UEDA^{a), b)}, and Kimitsuna WATANABE^a)** ^a) Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo (7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan)* ^b) Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo (7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan)

It has been known that RNA has a lot of modified nucleosides which are post-transcriptionally introduced by specific enzymes. Modified nucleosides play an important role in the RNA function. In the case of transfer RNA, modified uridine at the first letter of anticodon is thought to participate in the selection of cognate codon so as to ensure the accurate translation. In this report, we tried to detect unknown species of modified uridine from bovine mitochondrial tRNAs by ESI/iontrap mass spectrometer with a new method for derivatizing the modified uridines with CMC [N-cyclohexyl-N′-b-(4-methylmorpholinium)ethylcarbodiimide].

Key words: MALDI-TOF/MS, Peptide-mass fingerprinting, Proteome, Photoreceptor, Retina, 2D-PAGE, In-gel digestion