JMSSJ On-line

JMSSJ On-line, Vol. 47 (1999) No. 3, pp. 183-190

Analysis of Rat Liver Proteins by Two-dimensional Polyacrylamide Gel Electrophoresis and Mass Spectrometry		>> Full Text PDF >> References
*Toshiyuki KOSAKA^a), Sachiko HORIUCHI^a), and Takemichi NAKAMURA^a)** ^a) Analytical and Metabolic Research Laboratories, Sankyo Co., Ltd. (2-58 Hiromachi 1-chome, Shinagawa-ku, Tokyo 140-8710, Japan)*

Proteome analysis exploits advanced two-dimensional gel electrophoresis (2-DE) technology and efficient methods for protein identification supported by modern mass spectrometry, and now becomes a very common tool for functional genomics, drug-target discovery, etc. In this paper, a procedure of proteome analysis is described with an example of the analysis of varied protein expression related to a disease. The protein expression patterns in Zucker diabetic fatty (ZDF) rat, which is a model of non-insulin-dependent diabetes mellitus, and lean ZDF control rat livers were displayed with 2-DE and the differences were analyzed. Two proteins which showed significant change in the protein expression, were identified by a combination of in-gel digestion, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and database search with peptide-mass fingerprinting.

Key words: Proteome, Two-dimensional gel electrophoresis, MALDI-TOF, Peptide-mass fingerprinting, FT-ICR