JMSSJ On-line

JMSSJ On-line, Vol. 48 (2000) No. 1, pp. 26-31

Differentiation between N-Acetylchondrosine and N-Acetyldermosine by Matrix-Assisted Laser Desorption Ionization Time of Flight Post Source Decay Mass Spectrometry		>> Full Text PDF >> References
*Mamoru OHASHI,^a) Masami ARAKI,^a) Takumi SAISU,^b) Takuji NABETANI,^b) and Haruki NIWA^b)** ^† This paper is dedicated to the late Professor Akira TATEMATSU. ^a) Department of Materials Science, Kanagawa University (Tsuchiya, Kanagawa 259-1293, Japan)* ^b) Department of Applied Physics and Chemistry, The University of Electro-Communications (Chofu, Tokyo 182-8585, Japan)

Matrix assisted laser desorption ionization (MALDI)-time of flight (TOF) post source decay (PSD) mass spectrometry has been applied to the characterization of a pair of stereoisomers, N-acetylchondrosine (1) and N-acetyldermosine (2). The stereoisomers were distinguished from each other on the basis of the PSD spectra of [M - H + 2Na]⁺ as the precursor ions. A fragment ion at m/z 145 was abundant in the spectra of the latter compound, while negligible in the former. Similar behavior was observed in the cases of potassium salts of these compounds. The characteristics of MALDI-TOF-PSD spectra turned out to be quite similar to those of FAB-CID-MS/MS.

Key words: N-Acetylchondrosine, N-Acetyldermosine, MAL-DI-TOF, PSD, Glycosaminoglycans, Disaccharides