| Analysis of Carotenoids by FAB CID-MS/MS |
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*a) Graduate School of Pharmaceutical Sciences, Kyoto University (46-29 Yoshida-shimoadachi-cho, Sakyou-ku, Kyoto 606-8501, Japan) b) Kyoto Pharmaceutical University (5 Misasagi-nakauchicho, Yamashina-ku, Kyoto 607-8414, Japan) |
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| Fast atom bombardment collision induced dissociation mass spectrometry/mass spectrometry (FAB CID-MS/MS) spectra were investigated to clarify the relationship between the structure and the product ions of 28 different carotenoids. In the case of β-carotene, zeaxanthin, etc. having symmetrical structure, a characteristic ion was prominently observed at [M - 92]+. generated by elimination of toluene from the polyene chain and high intensity ions corresponding 14 mass units apart (m/z 91, 105, 119) at the low mass region appeared. On the other hand, in violaxanthin, etc. having epoxy group conjugated to the polyene was observed the pronounced characteristic ion at [M - 80]+.. Product ions formed by the cleavage at the C-C bond of the polyene chain were also noticeable. Further, these spectra revealed the characteristic ions from the end group. In the case of carotenoids with the antisymmetrical structure, the fragmentation patterns were characterized by the respective end groups. On the basis of the data obtained by this FAB CID-MS/MS technique, Frit-FAB LC/MS/MS was also applied successfully to the identification of several carotenoids from paprika oleoresin. | ||
| Key words: Carotenoids, FAB CID-MS/MS, Frit-FAB LC/MS/MS, Fragment ions | ||
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