JMSSJ On-line

JMSSJ On-line, Vol. 48 (2000) No. 2, pp. 150-154

Determination of Emedastine in Plasma by the MALDI-TOF MS		>> Full Text PDF >> References
*Yoichiro NIHASHI^a), Mitsuhiro ISHIDA^a), Fumio SAKAMOTO^a), and Yuichiro NAKADA^a)** ^a) Nippon-Organon Research & Development Laboratories (1-5-90 Tomobuchi-cho, Miyakojima-ku, Osaka 534-0016, Japan)*

Determination of low molecular weight compounds by MALDI-TOF MS was developed using emedastine, an anti-allergic drug, as model compound. It was found that α-cyano-4-hydroxycinnamic acid is suitable as matrix and a mixture of acetonitrile and water (7:3) as solvent for the measurement. Emedastine in rat plasma was extracted with mixture of hexane and chloroform (7:3). Calibration curve of emedastine in rat plasma was linear over the range 5-100 ng/mL with coefficient of 0.997. It seems that MALDI-TOF MS is useful for the determination of a drug in plasma.

Key words: MALDI-TOF MS, Emedastine