| Simultaneous Determination Method of Emedastine and Its Metabolites in Human Plasma Using High-Performance Liquid Chromatography-Tandem Mass Spectrometry |
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*a) Research and Development Division, Nippon Organon K. K., (5-90 Tomobuchi-cho 1-chome, Miyakojima-ku, Osaka 534-0016, Japan) |
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| A simultaneous and high sensitivity determination method of emedastine and its major metabolites, 5- and 6-hydroxy emedastine, in human plasma was developed. Plasma samples were extracted by Extrelut-1 column. Extracts were separated by reverse phase C18 column under stepwise gradient conditions. An atmospheric pressure chemical ionization interface was used in positive ionization mode. The analytes were detected using a tandem mass spectrometer operated selected reactant monitoring mode. The method was linear over the range 50-5,000 pg/mL (γ=0.999). This method is suitable for therapeutic drug monitoring of unchanged drug and its major metabolites. | ||
| Key words: Emedastine, LC/MS/MS, APCI, Human plasma | ||
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