| The Forefront of Amino Acid Sequencing by Mass Spectrometry; Scaling Down and Automation |
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* Central Research Laboratories, Ajinomoto Co., Inc. (1-1 Suzuki-cho, Kawasaki 210-8681, Japan) |
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| Proteomics is an effective approach for understanding of biology with the global change in protein expression as visualized by two-dimensional gel electrophoresis and characterized by mass spectrometry. For proteomics to be widely adopted, a robust technology must be established that allows the large-scale discovery research needed for an exhaustive approach to protein science. And a drastic increase in the rapidity and sensitivity of protein identification is needed for proteome analysis. In this paper, I summarize methodology of rapid and micro amino acid sequencing and recent trends toward automation in instrumentation and software for protein identification by mass spectrometry. | ||
| Key words: Protein identification, MALDI-TOFMS, Nano-ESIMS, LC/MS/MS, Automation, Data base research, Proteomics | ||
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