| Stereoisomer Discrimination of Some Amino Sugars: Chiral Recognition FAB Mass Spectrometry Coupled with the Enantiomer-Labeled Host Method |
>> Full Text PDF
>> References |
|
*a) Materials Analysis Center, The Institute of Scientific and Industrial Research, Osaka University (8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan) b) Department of Applied Chemistry, Kansai University (Suita, Osaka 564-8680, Japan) c) Department of Environmental Sciences, Faculty of Science, Osaka Women's University (2-1 Daisen-cho, Sakai, Osaka 590-0035, Japan) |
||
| FAB mass spectrometry coupled with the host-guest complexation method was used to distinguish some amino sugars (1-8) including glucosamine, galactosamine, and mannosamine, etc. The method characteristically used a 1:1 mixture of the chiral crown ether host whose enantiomer was isotopically labeled. Diastereomer differentiation of a given amino sugar salt (G+) was simply measured with a given host pair (A or B) (HRRRR:HSSSS-d6=1:1) from the peak intensity ratio of the two diastereomeric host-guest complex ions as I[(HRRRR+G)+]/I[(HSSSS-d6+G)+]≡IRIS (abbreviation). The stereoisomer differentiation was performed by the IRIS values in the range from 0.4 to 2.0 (for the use of the host pair A) and from 0.4 to 3.4 (for the use of the host pair B). The five underivatized amino sugar hydrochloride salts (1-5) were successfully differentiated using the present chiral recognition FAB mass spectrometry. The stereoisomer-(13C)-labeled guest method was also presensed to evaluate their relative complexation abilities. | ||
| Key words: Chiral recognition, Host-guest complex, Amino sugar, Chiral crown ether, Deuterium labeling, Carbon-13 labeling, FAB mass spectrometry | ||
| [ Full Text PDF ] [ References ] |
| © COPYRIGHT by The Mass Spectrometry Society of Japan. All Rights Reserved. |