JMSSJ On-line

JMSSJ On-line, Vol. 51 (2003) No. 1, pp. 256-259

Development of a Novel Bacterial Biodegradation Test Method of Non-ionic Surfactants using ¹⁸O-Labelled Water		>> Full Text PDF >> References
*Atsushi SHIBATA,^a) Hiroaki SATO,^b) Hiromichi YOSHIKAWA,^c) and Hiroto TAMURA^a),d)** ^a)Faculty of Agriculture, Meijo University (Nagoya 468-8502, Japan) ^*b)Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology (AIST) (Tsukuba 305-8569, Japan) ^c)Faculty of Engineering, Kyushu Kyoritsu University (Kitakyushu 807-8585, Japan) ^d)Agricultural High-Tech Research Center, Meijo University (Nagoya 468-8502, Japan)

In order to investigate the oxidative biodegradation mechanisms of octylphenol polyethoxylate (OPEO) by matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS), fundamental conditions for the bacterial biodegradation test method using ¹⁸O-labelled water as an incubation medium were examined. Since ¹⁸O-labelled water is a valuable reagent, reduction of incubation medium down to 200 µL could be achieved. Furthermore, to recover OPEO degradation intermediates from a minimum amount of the medium containing basal salt, a simple solid-liquid extraction procedure was introduced. The combination of the biodegradation test using ¹⁸O-labelled water and MALDI-MS measurement thus could provide a conclusive biodegradation mechanism by the observation of incorporation of ¹⁸O atoms into the carboxyl group of OPEC during the biodegradation process.

Key words: Biodegradation test, Alkylphenol polyethoxylate, MALDI-MS, ¹⁸O-Labeled water, Psudomonas putida

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