| Structural Analysis of Oligosaccharides by In-source Decay/Post-source Decay Experiments and Relative Ion Abundance Analysis of MALDI-MS |
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Department of Chemistry, School of Science, University of Tokyo (7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan) E-mail address: yamagaki@chem.s.u-tokyo.ac.jp |
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| Lewis-type trisaccharides were analyzed by MALDI-MS. The post-source decay spectra of Lewisx(Lex) and Lewisa (Lea) trisaccharides differed each other because of the β-elimination at C-3 in the N-acetyl glucosamine of the reducing-end of Lex. The mechanism of the β-elimination in N-acetyl glucosamine of Lex was analyzed by combined in-source decay/post-source decay (ISD/PSD) measurements. The combined ISD/PSD measurements were powerful tool for the fine analysis of the mechanism of the post-source decay of the oligosaccharides. ISD/PSD measurements were also useful for the assignments of the chemical species of PSD ions. | ||
| Key words: Oligosaccharide, MALDI-MS, Post-source decay (PSD), In-source decay (ISD), Structural isomers | ||
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