JMSSJ On-line

JMSSJ On-line, Vol. 51 (2003) No. 5, pp. 538-541

Investigation of Water Quality for Proteome Analysis		>> Full Text PDF >> References
*Hiroya KUMAI ^a) and Naoe ISHII ^a)** ^a)Laboratory Water Div., Nihon Millipore K.K. (Mita Kokusai Bldg., Mita 1-4-28, Minato-ku, Tokyo 108-0073, Japan)*

Proteome analysis is an effective means to identify molecules which have important roles in cells or organs. In this analysis, the electrophoresis is indispensable technology to separate proteins in details. However, the lateness of separation speed is a bottleneck for high throughput. We tried the electrophoresis with gels and buffers prepared by three type waters. As a comparison of the water, the purity shows possibility to contribute to acceleration of the electrophoresis speed. On the other hand, MALDI-TOF/MS or LC/MS analysis are performed after sample separation by 2D gel electrophoresis. In order to perform sensitive analysis especially by LC/MS, the high purity of the water used for preparing reagents is required. To estimate the effect of water, HPLC and LC/MS analysis were performed on commercially available bottled HPLC-grade waters and on water polished using Milli-Q water systems which have different purification technologies. As a result, UV oxidation on the purity of the ultra pure water was effective to reduce LC/MS backgrounds and noises.

Key words: Electrophoresis, LC/MS, TOC, Ultra pure water