JMSSJ On-line

JMSSJ On-line, Vol. 51 (2003) No. 5, pp. 550-553

Post-source Decay Analysis of Novel Heterogeneous Sugar Branched Cyclodextrins by Using Matrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry		>> Full Text PDF >> References
*Tohru YAMAGAKI,^a) Ken-ichi HAMAYASU,^b)Hitoshi HASHIMOTO,^b) and Hiroshi NAKANISHI,^c)** ^*a) Department of Chemistry, School of Science, The University of Tokyo (7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan)E-mail: yamagaki@chem.s.u-tokyo.ac.jp ^b) Bio Research Corporation of Yokohama, (Techno-Core 5F, Yokohama-Kanazawa High-thch Center, 1-1-1 Fuku-ura, Kanazawa-ku, Yokohama 236-0004, Japan) ^c) Biological Information Research Center, National Institute of Advanced Industrial Science and Technology, (1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan)

Novel heterogeneous cyclodextrin (CyD) compounds of 6-O-(4-O-α-D-glucuronyl-α-D-glucosyl)cyclomaltoheptaose (GlcU-Glc-βCyD) and 6-O-(N-acetyl-β-D-glucosaminyl)cyclomaltoheptaose (GlcNAc-βCyD) were analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Since the branch sugar residues of these heterogeneous CyDs had different molecular weights from those of glucose residues, the product ions generated from the branch or CyD part could be distinguished by their PSD spectra. In the PSD spectrum of GlcU-Glc-βCyD, the product ions, [M - Glc₃₅ + Na] ⁺ , [M - GlcU + Na] ⁺ , and [M - GlcU - Glc₁₅ + Na] ⁺, were observed. The chemical species of [M - Glc₃₅ + Na] ⁺ was generated by the two-site cleavage of the glycosyl linkages in the CyD part without cleavage of the branch. In the PSD spectrum of GlcNAc-βCD, [M - Glc₁₅ + Na] ⁺ , and [M - GlcNAc - Glc₁₄ + Na] ⁺ ions were observed.

Key words: Cyclodextrin, Oligosaccharide, PSD, MALDI-MS

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