JMSSJ On-line, Vol. 52 (2004) No. 1, pp. 13-20
Reduction of Cu(II) and Riboflavin in DIOS Mass Spectrometry
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    Shoji OKUNO *a), Ryuichi ARAKAWA a),b), and Yoshinao Wada a),*c)

    *a)Japan Science and Technology Agency, Innovation Plaza Osaka, Wada Project Laboratory (3-1-10 Technostage, Izumi, Osaka 594-1144, Japan) b)Department of Applied Chemistry, Kansai University (3-3-35 Yamatecho, Suita, Osaka 564-8680, Japan) *c)Osaka Medical Center and Research Institute for Maternal and Child Health (840 Murodo-cho, Izumi, Osaka 594-1101, Japan)
Understanding ion-molecular reactions is suggested to be essential to delineating the mechanisms of matrix-assisted laser desorption/ionization (MALDI) and related ionization methods. To examine the implications of matrix-analyte reactions in the reduction observed in MALDI, copper(II) chloride and riboflavin were analyzed by desorption/ionization on porous silicon (DIOS) mass spectrometry. This system does not require a matrix for soft laser desorption/ionization. The two susbstances were reduced by DIOS as well, and the reduction of riboflavin was accelerated using water as a solvent, as reported in MALDI. The results indicated that target-analyte electron transfer occurs in LDI, although the possibility of matrix-analyte electron transfer in MALDI cannot be excluded.
Key words: Reduction, Electron transfer, DIOS (desorption/ionization on porous silicon), Copper dichloride, Riboflavin
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