JMSSJ On-line

JMSSJ On-line, Vol. 53 (2005) No. 1, pp. 1-6

Evaluation of Solution Phase Isoelectrofocusing as Part of Proteomics Strategies		>> Full Text PDF >> References
*Yanming AN,^a) Zongming FU,^a) and Catherine FENSELAU^a)** ^*a) Department of Chemistry & Biochemistry, University of Maryland (College Park, MD 20742, U.S.A.) and Greenebaum Cancer Center, University of Maryland Medical School (Baltimore, MD 21201, U.S.A.) e-mail: fenselau@umd.edu

Solution isoelectrofocusing has been evaluated for prefractionation of nuclear proteins from human cancer MCF-7 cells. Two separation devices were exercised using commercial polyacrylamide membranes, which carry embedded immobiline mixtures to establish pH boundaries between chambers. Protein recovery was quantified and 2-D gel electrophoresis was used to evaluate resolution. Solution isoelectrofocusing provides satisfactory separations and decreases the complexity of the protein mixture, however rates of recovery need to be improved.

Key words: Proteomics, Isoelectrofocusing, Nuclear proteins, Multi-compartment electrolyzer, MALDI-TOF