| Optimization of Nano-Liquid Chromatography Coupled with Mass Spectrometry Systems for Proteomics |
>> Full Text PDF
>> References |
|
1 Institute for Advanced Biosciences, Keio University, Tsuruoka, YAMAGATA, JAPAN 2 PRESTO, Japan Science and Technology Agency, Kawaguchi, SAITAMA, JAPAN |
||
| Current proteomics technologies based on miniaturized on-line liquid chromatography (LC) coupled with mass spectrometry (MS) allow us to analyze protein expression profiling with a high throughput. Compared to genome sequencing and microarray-based transcriptomics, however, proteomics is still under development to achieve coverage of the entire proteome. In this commentary, the optimization of proteomic nanoLC/MS systems is described. In addition, future development of the analytical systems to achieve the coverage of the entire proteome is discussed. | ||
| Key words: NanoLC-MS, Proteomics, LC fractionation, LC optimization, Mass accuracy | ||
| [ Full Text PDF ] [ References ] |
| © COPYRIGHT by The Mass Spectrometry Society of Japan. All Rights Reserved. |