| Sample Preparation for the Systematic Analysis of a Protein-Protein Interaction Network |
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Biological Information Research Center (BIRC), National Institute of Advanced Industrial Science and Technology (AIST), TOKYO, JAPAN |
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| Functional proteomics aims to determine the cellular functions of genes directly at the protein level, and includes the comprehensive analysis of proteinprotein interactions by affnity-based isolation of protein complexes. Mass spectrometry (MS) is a powerful method for the identification of proteins. To enhance the sensitivity and resolution of the MS/MS analysis, we developed a novel direct nanoflow liquid chromatography/MS/MS system. Furthermore, to enhance the sensitivity of the whole analysis system, we optimized the conditions of sample preparation. In this review, we describe the pull-down procedures. | ||
| Key words: Proteomics, Proteinprotein interaction, Pull down, Epitope tag, Direct nanoflow LC/MS/MS | ||
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