| H/D Exchange Study of Macro-Protein Complexes by Mass Spectrometry |
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1 Center for Quantum Life Sciences, Hiroshima University, Higashi-Hiroshima, HIROSHIMA, JAPAN 2 Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima, HIROSHIMA, JAPAN |
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| The 70S ribosome of Escherichia coli, which plays a central role in peptide synthesis in cells, is a super-macro complex (2.7 MDa) comprising three RNA molecules and more than 50 proteins. We succeeded in measuring the hydrogen/deuterium exchange of 55 ribosomal proteins by matrix-assisted laser desorption/ionization time-offlight mass spectrometry. The results revealed the structural flexibility and solvent accessibility of individual proteins, and provided useful information on the structure-flexibility-function relationships of 70S ribosome. | ||
| Key words: 70S ribosome, MALDI mass spectrometry, H/D exchange, Structure-flexibility-function relationship | ||
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