JMSSJ On-line

JMSSJ On-line, Vol. 57 (2009) No. 3, pp. 145-151

Sample Preparation Methods for Large-Scale Analysis of Membrane Proteins Using Shotgun Proteomics		>> Full Text PDF >> References
Takeshi MASUDA¹ and Yasushi ISHIHAMA^{1, 2} ¹ Institute for Advanced Biosciences, Keio University, Tsuruoka, YAMAGATA, JAPAN ² PRESTO, Japan Science and Technology Agency, Chiyoda-ku, TOKYO, JAPAN

Shotgun proteomics based on liquid chromatography/mass spectrometry has been widely used to provide a large-scale view of the proteome, including protein expression, localization, modification, and protein-protein interaction. However, it is still challenging tasks to carry out membrane protein-targeted proteome analysis due to the hydrophobic nature of proteins with transmembrane domains. Here, we review current sample preparation techniques such as enrichment, solubilization, and digestion for membrane proteomics and focus on phase-transfer surfactant-based approaches recently developed by our group. We also discuss future developments for membrane proteome focused shotgun proteomics.

Key words: Shotgun proteomics, Membrane protein, LC/MS/MS, Sample preparation, Phase transfer surfactant