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Quantitative Profiling of Lipid Mediators by Liquid Chromatography/Mass Spectrometry -Development and Applications- |
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Department of Biochemistry and Molecular Biology, Faculty of Medicine, the University of Tokyo, Bunkyo-ku, TOKYO, JAPAN |
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| Lipid mediators, including eicosanoids and the platelet activating factor (PAF), are produced from common precursor phospholipids in mammalian tissues and cells. They play diverse roles in a variety of pathological and physiological contexts. For comprehensive lipid mediator profiling purposes, we have developed a liquid chromatography/mass spectrometry (LC-MS) multiplex quantitation method that covers eicosanoids and PAF. In this article, we describe the development of the LC-MS system, the sample pretreatment procedure optimized for this system, and application of lipid mediator profiling in macrophages and animal disease models. Also, we briefly introduce our perspective on label-free differential phospholipid analysis using ultra performance liquid chromatography/mass spectrometry (UPLC-MS). | ||
| Key words: Lipid mediator, Phospholipid, Quantitation, Lipidomics | ||
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