JMSSJ On-line

JMSSJ On-line, Vol. 59 (2011) No. 3, pp. 057-061

Novel Ion Extraction Method for Imaging Mass Spectrometry		>> Full Text PDF >> References
Jun AOKI,^{1, 3} Hisanao HAZAMA,^{2, 3} and Michisato TOYODA^{1, 3} ¹ Graduate School of Science, Osaka University, Toyonaka, OSAKA, JAPAN ² Graduate School of Enginnering, Osaka University, Suita, OSAKA, JAPAN ³ Japan Science and Technology Agency, CREST, Chiyoda-ku, TOKYO, JAPAN

We have developed a new ion extraction method for a stigmatic matrix-assisted laser desorption/ionization (MALDI) imaging time-of-flight (TOF) mass spectrometer. The conventional delayed extraction method is unsuitable for a stigmatic imaging TOF mass spectrometer because the ions disperse until extraction and the ion distribution is not preserved for imaging. In the proposed method, temporal and spatial focusing can be simultaneously achieved, enabling a high-fidelity image to be obtained with a high mass resolution. This method is expected to be applicable to cellular-level observations by imaging mass spectrometry.

Key words: Stigmatic imaging mass spectrometry, Ion extraction method